分子克隆实验指南

Molecular Cloning has served as the foundation of technical
expertise in labs worldwide for 30 years. No other manual has been
so popular, or so influential. Molecular Cloning, Fourth Edition,
by the celebrated founding author Joe Sambrook and new co-author,
the distinguished HHMI investigator Michael Green, preserves the
highly praised detail and clarity of previous editions and includes
specific chapters and protocols commissioned for the book from
expert practitioners at Yale, U Mass, Rockefeller University, Texas
Tech, Cold Spring Harbor Laboratory, Washington University, and
other leading institutions. The theoretical and historical
underpinnings of techniques are prominent features of the
presentation throughout, information that does much to help
trouble-shoot experimental problems.
For the fourth edition of this classic work, the content has
been entirely recast to include nucleic-acid based methods selected
as the most widely used and valuable in molecular and cellular
biology laboratories.
Core chapters from the third edition have been revised to
feature current strategies and approaches to the preparation and
cloning of nucleic acids, gene transfer, and expression analysis.
They are augmented by 12 new chapters which show how DNA, RNA, and
proteins should be prepared, evaluated, and manipulated, and how
data generation and analysis can be handled.
The new content includes methods for studying interactions
between cellular components, such as microarrays, next-generation
sequencing technologies, RNA interference, and epigenetic analysis
using DNA methylation techniques and chromatin immunoprecipitation.
To make sense of the wealth of data produced by these techniques, a
bioinformatics chapter describes the use of analytical tools for
comparing sequences of genes and proteins and identifying common
expression patterns among sets of genes.
Building on thirty years of trust, reliability, and authority,
the fourth edition of Molecular Cloning is the new gold
standard—the one indispensable molecular biology laboratory manual
and reference source.