黑暗笼罩的哥谭镇，暗夜里总在上演一幕幕追逐。布鲁斯·韦恩白天是社交宠儿、巨富青年，夜晚则是行走在夜间的蝙蝠侠，他利用自己的财富、智慧和高科技装备，致力于打击哥谭镇的犯罪。他的对手有珠宝大盗、美艳的猫女，玩世不恭、搞出各种致命恶作剧的小丑，失去爱人又被害成“急冻人”的研究员，曾是著名演员、欲找回被车祸损毁容貌而过量使用神奇面霜的“泥人”……每个坏人身上，都有一个令人唏嘘的故事，蝙蝠侠秉承他“不杀生”的原则，作为哥谭镇正义的化身，将一个个罪犯绳之以法送进监狱。

S4, Ep1 - The Terrible Trio
Three rich youths who commit armed robbery for fun are pursued by Batman as their irresponsible hobby escal ates into potentially worse deeds.
S4, Ep2 - Showdown
While the Dynamic Duo race to stop Ra's Al Ghul from performing a kidnapping, the wild west story of how one of his sons was opposed by the disfigured bounty hunter, Jonah Hex, is told.
S4, Ep3 - Catwalk
Catwoman is trying to go straight, but is recruited by The Ventriloquist as a jewel thief.
S4, Ep4 - A Bullet for Bullock
Det. Bullock reluctantly asks for Batman's assistance when he is convinced someone is trying to kill him.
S4, Ep5 - The Lion and the Unicorn
Red Claw kidnaps an old colleague of Alfred's from the British Secret Service, to gain access to a hidden missile silo.
The fourth and final volume of Batman: The Animated Series is a little uneven but still encompasses some great episodes. Start with "Over the Edge," a nightmarish scenario in which Commissioner Gordon dedicates himself to hunting down Batman following the death of his daughter, Batgirl. "Critters" is a tribute to Japanese monster movies, "Mad Love" recounts the origin of Harley Quinn, and "Legends of the Dark Knight" animates both a '50s-styled Batman adventure as well as a scene from Frank Miller's Return of the Dark Knight. This run of 24 episodes has a lot of Batgirl, too little Nightwing, and a lot of the new Robin, Tim Drake, whose origin is explained in "Sins of the Father." Drake gets a mostly solo adventure, Batgirl teams with Supergirl, and there's still a sense of fun, with goofy humor that includes an appearance by the Three Stooges as the Joker's henchmen. Guest voices include Sela Ward as Calendar Girl and Tippi Hedren, and Mark Hamill continues the excellent work as the Joker that created some early rumors about his taking the live-action role in the sequel to Batman Begins. By this time, Batman had become part of the Batman/Superman Hour, so viewers can choose the opening sequence for either that or the original animated series. That, as well as the unbalanced number of episodes in the seasons (e.g., the first season of the animated series was 60 episodes), is why Batman: The Animated Series was released in volumes rather than as single-season sets

由于急冻博士的妻子得了重症已在死亡边缘，不顾一切的急冻博士重返高咸市并将蝙蝠女掳走打算作为他妻子的活体器官供体，蝙蝠侠和罗宾必须在有限的时间内阻止急冻博士的器官移植手术。