影片改编自同名漫画，原著漫画讲述一个神秘的跟踪者名叫“缄默”，他似乎打算从远处暗杀蝙蝠侠。故事中有许多客串角色，其中包含了大量的蝙蝠侠反派，还有多位蝙蝠家族的成员以及和蝙蝠侠的老战友，超人。它还探索了蝙蝠侠和猫女之间的暧昧情愫。

高谭市接二连三发生绑架案，嫌疑人都指向了蝙蝠侠。蝙蝠侠（杰森·奥玛拉 Jason O'Mara 配音）和绿灯侠（贾斯汀·柯克 Justin Kirk 配音）分头找到真凶的线索，对方留下一个神秘仪器后自爆。为了解开仪器的秘密，二人出发寻找超人（艾伦·图代克 Alan Tudyk 配音）希望弄清真相。当他们正逼近事实时，仪器突然启动，原来这是某个外星入侵者设置的时空通道装置，大批外星人通过它开始侵略地球。与此同时，斯通博士也就神秘仪器展开调查，斯通博士的儿子维克（谢默·摩尔 Shemar Moore 配音）意外被仪器感染，死而复生化身拥有强大实力的超级战士。
史无前例的危机，蝙蝠侠、超人、绿灯侠、闪电侠（克里斯托弗·高哈姆 Christopher Gorham 配音）、黛安娜（米歇尔·莫娜汉 Michelle Monaghan 配音）相继卷入战斗……

Caitlin Fairchild, is a teenager who is offered a place in an institute for gifted children. She soon learns that the school isn't really a school, but rather a military project to turn children with a special genetic structure into super soldiers. After developing incredibly enhanced abilities, Caitlin rebels against the program that created her. But all is not what it appears; there are some in the military who want to help her, and a deadly enemy is actually a long lost sibling.