薇薇安（艾玛·汤普森 Emma Thompson 饰）是一位学识渊博的博士，让年轻的她没有想到的是，上帝竟然给对生活和学术充满了热情的自己开了一个天大的玩笑，经过医生的确证，她患上了卵巢癌，病情已经发展到了末期，几乎没有治愈的可能。
从悲伤、抗拒到最后的接受，薇薇安的内心犹如踏过了千山万水。尽管康复的希望渺茫，但她还是积极地接受了可能令她形销骨立的化疗治疗。在身体逐渐枯萎，头发接连落光的过程中，这位睿智的女性也不忘对自己的前半生进行捉摸和思考，她在有限的生命中看到了无限，最终调整好了心态，以平静之姿态，面对必然到来之死亡。

The action of the play takes place during the final hours of Dr Vivian Bearing, a university professor of English, dying of ovarian cancer. She recalls the initial diagnosis of Stage IV metastatic ovarian cancer from her oncologist, Dr Harvey Kelekian. Dr Kelekian then proposes an experimental chemotherapeutic treatment regimen consisting of eight rounds at full dosage. Vivian agrees to the treatment.
Over the course of the play, Vivian reflects on her life through the intricacies of the English language, especially the use of wit in the metaphysical poetry of John Donne. Throughout the play, she recites Donne's Holy Sonnet X, "Death Be Not Proud," while reflecting upon her condition. (In the revised edition of John Donne's Holy Sonnets, "If Poysonous Mineralls" and "Death Be Not Proud" are sonnets V and VI, respectively.) As a professor, she has a reputation for rigorous teaching methods. She has lived her life alone, is unmarried and without children, her parents are deceased, and she has no emergency contact.
Vivian recalls undergoing tests by various medical technicians and being the subject of grand rounds. She remembers sharing a love of language and books with her father. She flashes back to her experiences as a student of Dr E. M. Ashford, an expert on John Donne. Bearing later finds herself under the care of Dr Jason Posner, an oncology research fellow who has taken her class on John Donne. At the hospital, she recognizes that doctors are interested in her for her research value and, like her, tend to ignore humanity in favor of knowledge. Gradually, she realizes that she would prefer kindness to intellectualism.
Vivian reaches the end stage in extreme pain as Susie Monahan, a nurse at the medical centre, offers Vivian compassion and discusses with her the option of exercising her final option, "do not resuscitate" (DNR), in case of a severe decline in her condition. Vivian decides to mark the DNR option. Dr Ashford, in town for her great-grandson's birthday, visits the hospital after learning of Vivian's cancer. She comforts her and offers to read a Donne sonnet, but Vivian, scarcely conscious, declines. Instead, Ashford reads from Margaret Wise Brown's The Runaway Bunny, which she had bought for her great-grandson.
When Vivian flatlines, Jason tries to resuscitate her, and calls in a medical team to administer CPR. Susie tries to stop him, pointing out the DNR instruction. Jason eventually realizes his mistake and calls for the CPR team to stop. The play ends as Vivian, unclothed after her death, walks from her hospital bed "toward a little light".