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基因工程原理(上册) 豆瓣
作者: 吴乃虎 出版社: 科学出版社 2001 - 3
《基因工程原理(上册)(第2版)》是在第一版的基础上,吸收了本学科的新进展,增加了大量的新内容,重新审订、编写而成。全书共十二章,分上下两册,书末附有基因工程名词术语解释及索引。《基因工程原理(上册)(第2版)》由三个有机联系的部分组成。第一部分论述基因工程赖以创立的理论及技术基础,重点介绍基因研究的发展及基因的现代概念;基因研究与基因工程的相互依赖关系;基因操作主要技术的基本原理;与基因克隆有关的上系列核酸酶的生化特性和在DNA重组中的应用等。第二部分由第四章至第八章组成,系《基因工程原理(上册)(第2版)》的核心。它详细地叙述了基因工程学所涉及的主要内容,包括各类分予克隆载体的构建、特点与应用;基因文库的构建、目的基因的分离与鉴定;克隆基因的表达与调控以及真核基因在大肠杆菌中表达的原理、方法及实例等。第三部分即《基因工程原理(上册)(第2版)》的后四章,主要论述基因工程实际应用方面的内容。它着重叙述高等植物及哺乳动物基因工程的研究目标、现状与进展,以及重组DNA技术在临床医学、农业生产、食品工业、化学制剂等若干重要领域的实际应用情况。《基因工程原理(上册)(第2版)》是一部有自己特色、体系新颖、基础理论与实际应用并重的基因工程学术专著。在内容的安排上注重科学性、先进性、系统性和条理性。
2019年4月12日 已读
不知道会不会出第三版了,毕竟超20年了。了解了一遍重组DNA的流程,粗略的凝胶电泳,内切限制酶和三个载体有了一点概念,细节的原理解说和优缺点说明都很扼要,不过读一遍完全没记住什么东西,这会儿脑子里就剩PCR和λ噬菌体了……
Biology 专业书 吴乃虎 基因 基因工程
Molecular Biology of the Cell, Fourth Edition 豆瓣
作者: Bruce Alberts / Alexander Johnson 出版社: Garland 2002 - 3
Molecular Biology of the Cell is the classic in-depth text reference in cell biology. By extracting the fundamental concepts from this enormous and ever-growing field, the authors tell the story of cell biology, and create a coherent framework through which non-expert readers may approach the subject. Written in clear and concise language, and beautifully illustrated, the book is enjoyable to read, and it provides a clear sense of the excitement of modern biology. Molecular Biology of the Cell sets forth the current understanding of cell biology (completely updated as of Autumn 2001), and it explores the intriguing implications and possibilities of the great deal that remains unknown.
The hallmark features of previous editions continue in the Fourth Edition. The book is designed with a clean and open, single-column layout. The art program maintains a completely consistent format and style, and includes over 1,600 photographs, electron micrographs, and original drawings by the authors. Clear and concise concept headings introduce each section. Every chapter contains extensive references. Most important, every chapter has been subjected to a rigorous, collaborative revision process where, in addition to incorporating comments from expert reviewers, each co-author reads and reviews the other authors' prose. The result is a truly integrated work with a single authorial voice.</P>
Molecular Biology of the Cell 豆瓣
作者: Bruce Alberts / Alexander Johnson 出版社: Garland Science 2008 - 1
For nearly a quarter century, "Molecular Biology of the Cell" has been the leading cell biology textbook. This tradition continues with the new Fifth Edition, which has been completely revised and updated to describe our current, rapidly advancing understanding of cell biology. To list but a few examples, a large amount of new material is presented on epigenetics; stem cells; RNAi; comparative genomics; the latest cancer therapies; apoptosis (now its own separate chapter); and cell cycle control and the mechanics of M phase (now integrated into one chapter). The hallmark features of "Molecular Biology of the Cell" have been retained, such as its consistent and comprehensive art program, clear concept headings, and succinct section summaries. Additionally, in response to extensive feedback from readers, the Fifth Edition now includes several new features. It is now more portable. Chapters 1-20 are printed and Chapters 21-25, covering multicellular systems, are provided as PDF files on the free Media DVD-ROM which accompanies the book. And for the first time, "Molecular Biology of the Cell" now contains end-of-chapter questions. These problems, written by John Wilson and Tim Hunt, emphasize a quantitative approach and the art of reasoning from experiments, and they will help students review and extend their knowledge derived from reading the textbook. The Media DVD-ROM, which is packaged with every copy of the book, contains PowerPoint[registered] presentations with all of the figures, tables and micrographs from the text (available as JPEGs too). Also included is the Media Player, which plays over 125 movies - animations, videos, and molecular models - all with voice-over narration. A new reader-friendly feature is the integration of media codes throughout the text that link directly to relevant videos and animations. The Media DVD-ROM holds the multicellular systems chapters (21-25) of the text as well. By skillfully extracting the fundamental concepts from this enormous and ever-growing field, the authors tell the story of cell biology, and thereby create a coherent framework through which readers may approach and enjoy this subject that is so central to all of biology. There is also a reference edition of "Molecular Biology of the Cell, Fifth Edition" (ISBN 978-0-8153-4111-6) that contains Chapters 1-25 entirely in printed format.
基因工程原理(下册)(第二版) 豆瓣
作者: 吴乃虎 出版社: 科学出版社 2001 - 9
《基因工程原理(下)》是在第一版的基础上,吸收了本学科的新进展,增加了大量的新内容,重新审订、编写而成。《基因工程原理(下)》共十二章,分上下两册,书末附有基因工程名词术语解释及索引。《基因工程原理(下)》由三个有机联系的部分组成。第一部分论述基因工程赖以创立的理论及技术基础,重点介绍基因研究的发展及基因的现代概念;基因研究与基因工程的相互依赖关系;基因操作主要技术的基本原理;与基因克隆有关的一系列核酸酶的生化特性和在DNA重组中的应用等。第二部分由第四章至第八章组成,系《基因工程原理(下)》的核心。它详细地叙述了基因工程学所涉及的主要内容,包括各类分子克隆载体的构建、特点与应用;基因文库的构建、目的基因的分离与鉴定;克隆基因的表达与调控以及真核基因在大肠杆菌中表达的原理、方法及实例等。第三部分即《基因工程原理(下)》的后四章,主要论述基因工程实际应用方面的内容。它着重叙述高等植物及哺乳动物基因工程的研究目标、现状与进展,以及重组DNA技术在临床医学、农业生产、食品工业、化学制剂等若干重要领域的实际应用情况。